| Preface |
|
vii | |
| Contributors |
|
xi | |
| 1 Aerobic Granulation at Different Carbon Sources and Concentrations |
|
1 | |
|
|
|
|
|
|
1 | |
|
1.2 Aerobic Granulation with Acetate and Glucose |
|
|
2 | |
|
1.2.1 Microscopic Observation of Aerobic Granulation |
|
|
2 | |
|
|
|
2 | |
|
1.2.1.2 Formation of Compact Aggregates after Operation for One Week |
|
|
2 | |
|
1.2.1.3 Formation of Granular Sludge after Operation for Two Weeks |
|
|
3 | |
|
1.2.1.4 Appearance of Mature Granules after Operation for Three Weeks |
|
|
4 | |
|
1.2.2 Characteristics of Glucose- and Acetate-Fed Aerobic Granules |
|
|
5 | |
|
|
|
5 | |
|
1.2.2.2 Sludge Settleability |
|
|
5 | |
|
1.2.2.3 Granule Physical Strength and Biomass Density |
|
|
7 | |
|
1.2.2.4 Cell Surface Hydrophobicity |
|
|
7 | |
|
1.2.2.5 Microbial Activity |
|
|
7 | |
|
1.2.2.6 Storage Stability of Aerobic Granules |
|
|
7 | |
|
1.3 Aerobic Granulation on Other Carbon Sources |
|
|
9 | |
|
1.4 Aerobic Granulation at Different COD Concentrations |
|
|
9 | |
|
1.4.1 Effect of COD Concentration on the Properties of Aerobic Granules |
|
|
10 | |
|
1.4.2 Effect of COD Concentration on the Reactor Performance |
|
|
15 | |
|
1.5 Aerobic Granulation at Different Substrate N/COD Ratios |
|
|
15 | |
|
1.5.1 Effect of N/COD Ratio on the Properties of Aerobic Granules |
|
|
16 | |
|
1.5.2 Effect of N/COD Ratio on Population Distribution |
|
|
18 | |
|
|
|
20 | |
|
|
|
20 | |
|
2 Aerobic Granulation at Different Shear Forces |
|
|
25 | |
|
|
|
|
|
|
25 | |
|
2.2 Aerobic Granulation at Different Shear Forces |
|
|
26 | |
|
2.3 Effect of Shear Force on Granule Size |
|
|
28 | |
|
2.4 Effect of Shear Force on Granule Morphology |
|
|
28 | |
|
2.5 Effect of Shear Force on Biomass Settleability |
|
|
31 | |
|
2.6 Effect of Shear Force on the Production of Cell Polysaccharides |
|
|
32 | |
|
2.7 Effect of Shear Force on Cell Hydrophobicity |
|
|
33 | |
|
|
|
34 | |
|
|
|
35 | |
|
3 Aerobic Granulation at Different SBR Cycle Times |
|
|
37 | |
|
|
|
|
|
|
37 | |
|
3.2 Effect of Cycle Time on Aerobic Granulation |
|
|
37 | |
|
3.3 Effect of Cycle Time on Properties of Aerobic Granules |
|
|
41 | |
|
|
|
49 | |
|
|
|
49 | |
|
4 Aerobic Granulation at Different Settling Times |
|
|
51 | |
|
|
|
|
|
|
51 | |
|
4.2 Effect of Settling Time on the Formation of Aerobic Granules |
|
|
52 | |
|
4.3 Effect of Settling Time on the Settleability of Sludge |
|
|
54 | |
|
4.4 Effect of Settling Time on Cell Surface Hydrophobicity |
|
|
55 | |
|
4.5 Effect of Settling Time on Production of Extracellular Polysaccharides |
|
|
56 | |
|
4.6 Effect of Settling Time on Microbial Activity of Aerobic Granules |
|
|
57 | |
|
4.7 Accumulation of Polyvalent Cations in Aerobic Granules |
|
|
58 | |
|
4.8 Effect of Shift of Settling Time on Aerobic Granulation |
|
|
60 | |
|
4.9 Effect of Settling Time on Microbial Population |
|
|
62 | |
|
4.10 Rationale Behind Settling Time-Initiated Aerobic Granulation |
|
|
62 | |
|
|
|
65 | |
|
|
|
65 | |
|
5 Roles of SBR Volume Exchange Ratio and Discharge Time in Aerobic Granulation |
|
|
69 | |
|
|
|
|
|
|
69 | |
|
5.2 The Role of SBR Volume Exchange Ratio in Aerobic Granulation |
|
|
70 | |
|
5.3 Effect of Volume Exchange Ratio on Aerobic Granulation |
|
|
71 | |
|
5.4 Effect of Volume Exchange Ratio on Sludge Settleability |
|
|
73 | |
|
5.5 Effect of Volume Exchange Ratio on Production of Extracellular Polysaccharides |
|
|
75 | |
|
5.6 Effect of Volume Exchange Ratio on Calcium Accumulation in Aerobic Granules |
|
|
75 | |
|
5.7 Volume Exchange Ratio Is a Selection Pressure for Aerobic Granulation |
|
|
76 | |
|
5.8 Effect of Discharge Time on Formation of Aerobic Granules |
|
|
78 | |
|
5.9 Effect of Discharge Time on Settleability of Bioparticles |
|
|
79 | |
|
5.10 Effect of Discharge Time on Cell Surface Hydrophobicity |
|
|
82 | |
|
5.11 Effect of Discharge Time on Production of Extracellular Polysaccharides |
|
|
82 | |
|
|
|
83 | |
|
|
|
84 | |
|
6 Selection Pressure Theory for Aerobic Granulation in Sequencing Batch Reactors |
|
|
85 | |
|
|
|
|
|
|
85 | |
|
6.2 Is Aerobic Granulation Inducible? |
|
|
86 | |
|
6.3 Earlier Understanding of Aerobic Granulation |
|
|
86 | |
|
6.4 Brief Review of Parameters Contributing to Aerobic Granulation |
|
|
87 | |
|
6.4.1 Substrate Composition and Loading |
|
|
88 | |
|
6.4.2 Hydrodynamic Shear Force |
|
|
88 | |
|
6.4.3 Feast-Famine Regimen |
|
|
89 | |
|
|
|
89 | |
|
|
|
89 | |
|
6.4.6 Reactor Configuration |
|
|
90 | |
|
6.4.7 Solids Retention Time |
|
|
90 | |
|
|
|
90 | |
|
|
|
91 | |
|
|
|
92 | |
|
|
|
92 | |
|
6.5 Main Selection Pressures of Aerobic Granulation |
|
|
92 | |
|
6.6 A Selection Pressure Theory for Aerobic Granulation in SBRs |
|
|
93 | |
|
6.7 Failure of Aerobic Granulation in Continuous Microbial Culture |
|
|
97 | |
|
6.8 Upscaling Aerobic Granular Sludge SBRs |
|
|
100 | |
|
6.9 Prediction of Settling Velocity of Bioparticles |
|
|
102 | |
|
|
|
107 | |
|
|
|
107 | |
|
7 Growth Kinetics of Aerobic Granules |
|
|
111 | |
|
|
|
|
|
|
111 | |
|
7.2 A Simple Kinetic Model for the Growth of Aerobic Granules |
|
|
112 | |
|
7.2.1 Growth of Aerobic Granules at Different Organic Loading Rates |
|
|
113 | |
|
7.2.2 Growth of Aerobic Granules at Different Shear Forces |
|
|
114 | |
|
7.2.3 Growth of Aerobic Granules at Different Substrate N/COD Ratios |
|
|
116 | |
|
7.3. Effect of Surface Loading on Kinetic Behavior of Aerobic Granules |
|
|
117 | |
|
7.3.1 Effect of Surface Loading on Growth Rate |
|
|
117 | |
|
7.3.2 Effect of Surface Loading on Substrate Biodegradation Rate |
|
|
118 | |
|
7.3.3 Relationship of Surface Growth Rate to Substrate Biodegradation Rate |
|
|
120 | |
|
7.4 Substrate Concentration-Associated Kinetic Behaviors of Aerobic Granules |
|
|
123 | |
|
7.5 A General Model for Aerobic Granular Sludge SBR |
|
|
124 | |
|
7.5.1 Description of Substrate Utilization |
|
|
125 | |
|
7.5.2 Description of Oxygen Transfer |
|
|
125 | |
|
7.5.3 Description of Diffusion of Substance |
|
|
126 | |
|
7.5.4 Description of Biological Reactions |
|
|
128 | |
|
|
|
128 | |
|
|
|
128 | |
|
8 Diffusion of Substrate and Oxygen in Aerobic Granules |
|
|
131 | |
|
Yong Li, Zhi-Wu Wang, and Yu Liu |
|
|
|
|
|
131 | |
|
8.2 Size-Dependent Kinetic Behaviors of Aerobic Granules |
|
|
132 | |
|
8.3 Description of Diffusion Resistance in Aerobic Granules |
|
|
133 | |
|
8.4 Simulation of Mass Transfer in Aerobic Granules |
|
|
135 | |
|
|
|
136 | |
|
8.4.2 Substrate Profile in Aerobic Granules with Different Radiuses |
|
|
139 | |
|
8.4.3 Oxygen Profiles in Aerobic Granules with Different Radiuses |
|
|
139 | |
|
8.4.4 Diffusion Profiles of Substrate in Aerobic Granules at Different Bulk Substrate Concentrations |
|
|
139 | |
|
8.4.5 Diffusion Profiles of Dissolved Oxygen in Aerobic Granules at Different Substrate Concentrations |
|
|
141 | |
|
8.4.6 Prediction of Bulk Substrate Concentration in an Aerobic Granules Reactor |
|
|
143 | |
|
|
|
144 | |
|
|
|
145 | |
|
|
|
146 | |
|
9 The Essential Role of Cell Surface Hydrophobicity in Aerobic Granulation |
|
|
149 | |
|
|
|
|
|
|
149 | |
|
9.2 Cell Surface Hydrophobicity |
|
|
150 | |
|
9.2.1 What Is Hydrophobicity? |
|
|
150 | |
|
9.2.2 Cell Surface Property-Associated Hydrophobicity |
|
|
151 | |
|
9.2.2.1 Surface Properties of Amino Acids |
|
|
151 | |
|
9.2.2.2 Surface Properties of Proteins |
|
|
151 | |
|
9.2.2.3 Surface Properties of Polysaccharides |
|
|
151 | |
|
9.2.2.4 Surface Properties of Phospholipids |
|
|
151 | |
|
9.2.3 Determination of Cell Surface Hydrophobicity |
|
|
152 | |
|
9.3 The Role of Cell Surface Hydrophobicity in Aerobic Granulation |
|
|
152 | |
|
9.4 Factors Influencing Cell Surface Hydrophobicity |
|
|
156 | |
|
9.5 Selection Pressure-Induced Cell Surface Hydrophobicity |
|
|
160 | |
|
9.6 Thermodynamic Interpretation of Cell Surface Hydrophobicity |
|
|
161 | |
|
9.7 Enhanced Aerobic Granulation by Highly Hydrophobic Microbial Seed |
|
|
170 | |
|
|
|
176 | |
|
|
|
176 | |
| 10 Essential Roles of Extracellular Polymeric Substances in Aerobic Granulation |
|
181 | |
|
|
|
|
|
|
181 | |
|
10.2 Main Composition of EPS in Aerobic Granules |
|
|
181 | |
|
10.3 Major Factors Influencing EPS Production In Aerobic Granules |
|
|
183 | |
|
10.4 The Role of EPS in Aerobic Granulation |
|
|
185 | |
|
10.5 EPS-Enhanced Stability of Aerobic Granules |
|
|
187 | |
|
|
|
191 | |
|
|
|
191 | |
| 11 Internal Structure of Aerobic Granules |
|
195 | |
|
|
|
|
|
|
195 | |
|
11.2 Internal Structure of Aerobic Granules |
|
|
195 | |
|
11.2.1 Heterogeneous Structure of Aerobic Granules |
|
|
195 | |
|
11.2.2 Porosity of Aerobic Granules |
|
|
196 | |
|
11.2.3 Size-Dependent Internal Structure of Aerobic Granules |
|
|
197 | |
|
11.2.4 Structure Change of Aerobic Granules during Starvation |
|
|
198 | |
|
11.3 Biomass Distribution in Aerobic Granules |
|
|
199 | |
|
11.4 PS Distribution in Aerobic Granules |
|
|
201 | |
|
11.5 Distribution of Cell Surface Hydrophobicity in Aerobic Granules |
|
|
205 | |
|
11.6 Diffusion-Related Structure of Aerobic Granules |
|
|
206 | |
|
|
|
207 | |
|
|
|
207 | |
| 12 Biodegradability of Extracellular Polymeric Substances Produced by Aerobic Granules |
|
209 | |
|
|
|
|
|
|
209 | |
|
12.2 Biodegradability of EPS Extracted from Aerobic Granules |
|
|
210 | |
|
12.2.1 Biodegradability of EPS Extracted from Fresh Aerobic Granules |
|
|
210 | |
|
12.2.2 Biodegradability of EPS Extracted from Starved Aerobic Granules |
|
|
212 | |
|
12.2.3 Comparison of Biodegradability of Acetate and Extracted EPS |
|
|
212 | |
|
12.3 Biodegradation of Aerobic Granule-Associated EPS during Starvation |
|
|
214 | |
|
12.4 EPS Biodegradation in an Aerobic Granular Sludge SBR |
|
|
216 | |
|
12.5 Origin of Biodegradable Aerobic Granules-Associated EPS |
|
|
218 | |
|
|
|
220 | |
|
|
|
221 | |
| 13 Calcium Accumulation in Acetate-Fed Aerobic Granules |
|
223 | |
|
Zhi-Wu Wang, Yong Li, and Yu Liu |
|
|
|
|
|
223 | |
|
13.2 Effect of Calcium on Aerobic Granulation |
|
|
224 | |
|
13.3 Calcium Accumulation in Acetate-Fed Aerobic Granules |
|
|
225 | |
|
13.4 Chemical form of calcium in acetate-fed aerobic granules |
|
|
226 | |
|
13.5 Calcium Distribution in Acetate-Fed Aerobic Granules |
|
|
227 | |
|
13.6 Granule Size-Dependent CaCO3 Formation in Acetate-Fed Aerobic Granules |
|
|
227 | |
|
13.7 Mechanism of Calcium Accumulation in Acetate-Fed Aerobic Granules |
|
|
229 | |
|
13.7.1 Ionic Equilibrium of Carbonate Ion |
|
|
230 | |
|
13.7.2 Diffusion Kinetics in Aerobic Granules |
|
|
231 | |
|
13.7.3 Distribution of pH and CO32- in Acetate-Fed Aerobic Granules |
|
|
233 | |
|
13.7.4 Size-Associated Formation of CaCO3 in Acetate-Fed Aerobic Granules |
|
|
234 | |
|
|
|
235 | |
|
|
|
236 | |
|
|
|
236 | |
| 14 Influence of Starvation on Aerobic Granulation |
|
239 | |
|
Yu Liu, Zhi-Wu Wang, and Qi-Shan Liu |
|
|
|
|
|
239 | |
|
14.2 Positive Effect of Starvation on Aerobic Granulation |
|
|
240 | |
|
14.2.1 Observation of Aerobic Granulation in an SBR |
|
|
240 | |
|
14.2.2 Periodic Starvation in the SBR |
|
|
241 | |
|
14.2.3 Effect of Periodic Starvation on Cell Surface Hydrophobicity |
|
|
242 | |
|
14.3 Influence of Short Starvation on Aerobic Granules |
|
|
245 | |
|
14.3.1 Influence of Carbon and Nutrients Starvation on Cell Surface Property |
|
|
246 | |
|
14.3.2 Influence of Carbon and Nutrients Starvation on EPS Content |
|
|
250 | |
|
14.3.3 Influence of Carbon and Nutrients Starvation on Microbial Activity and Production |
|
|
250 | |
|
|
|
255 | |
|
|
|
255 | |
| 15 Filamentous Growth in an Aerobic Granular Sludge SBR |
|
259 | |
|
|
|
|
|
|
259 | |
|
15.2 Causes of Filamentous Growth in the Activated Sludge Process |
|
|
260 | |
|
15.2.1 Wastewater Composition |
|
|
260 | |
|
15.2.2 Substrate Availability |
|
|
260 | |
|
15.2.3 Dissolved Oxygen Concentration |
|
|
261 | |
|
15.2.4 Solids Retention Time (SRT) |
|
|
261 | |
|
15.2.5 Nutrient Deficiency |
|
|
262 | |
|
|
|
262 | |
|
15.2 Outgrowth of Filamentous Bacteria in Aerobic Granular Sludge SBRs |
|
|
262 | |
|
15.4 Causes of Filamentous Growth in Aerobic Granular Sludge SBRs |
|
|
267 | |
|
|
|
267 | |
|
15.4.2 Long Solids Retention Time in Aerobic Granular Sludge SBRs |
|
|
268 | |
|
15.4.3 Substrate Concentration and Concentration Gradients |
|
|
270 | |
|
15.4.4 Dissolved Oxygen Deficiency in Aerobic Granules |
|
|
272 | |
|
15.4.5 Nutrient Deficiency in Aerobic Granules |
|
|
276 | |
|
15.4.6 Temperature Shift in Aerobic Granular Sludge SBRs |
|
|
277 | |
|
15.4.7 Flow Patterns in Aerobic Granular Sludge SBRs |
|
|
278 | |
|
15.4.8 Length of Aerobic Feeding |
|
|
279 | |
|
15.5 Propagation Patterns of Filamentous Growth in Aerobic Granular Sludge SBRs |
|
|
280 | |
|
15.6 Control Strategy for Filamentous Growth |
|
|
282 | |
|
|
|
283 | |
|
|
|
283 | |
| 16 Improved Stability of Aerobic Granules by Selecting Slow-Growing Bacteria |
|
287 | |
|
|
|
|
|
|
287 | |
|
16.2 Improved Stability of Aerobic Granules by Selecting Slow-Growing Nitrifying Bacteria |
|
|
288 | |
|
16.3 Improved Stability of Aerobic Granules by Selecting Slow-Growing P- or Glycogen-Accumulating Organisms |
|
|
294 | |
|
16.4 Improved Stability of Aerobic Granules by Selecting Aged Aerobic Granules |
|
|
296 | |
|
|
|
296 | |
|
|
|
298 | |
| 17 Pilot Study of Aerobic Granulation for Wastewater Treatment |
|
301 | |
|
|
|
|
|
|
301 | |
|
17.2 Startup of Pilot-Scale Aerobic Granular Sludge SBRs |
|
|
302 | |
|
17.2.1 Comparison of Pilot- and Laboratory-Scale SBRs |
|
|
302 | |
|
17.2.2 Characteristics of Aerobic Granules Developed in Pilot- and Laboratory-Scale SBRs |
|
|
305 | |
|
17.2.2.1 Granule Size and Morphology |
|
|
305 | |
|
17.2.2.2 Settling Property |
|
|
305 | |
|
17.2.2.3 Physical Strength |
|
|
306 | |
|
17.2.2.4 Microbial activity |
|
|
306 | |
|
17.3 Startup of a Pilot-Scale SBR Using Stored Granules as Seed |
|
|
306 | |
|
17.4 Startup of a Pilot-Scale SBR Using Activated Sludge as Seed |
|
|
310 | |
|
|
|
311 | |
|
|
|
311 | |
| Index |
|
313 | |